In order to prepare and support space experiments, 2D and 3D clinostats are widely applied to study the influence of simulated weightlessness on biological systems. In order to evaluate the results a comparison between the data obtained in simulation experiments and in real microgravity is necessary. The authors are currently analyzing the gravity-dependent behavior of the protists Paramecium biaurelia (ciliate) and Euglena gracilis (photosynthetic flagellate) on these different experimental platforms. So far, first results are presented concerning the behaviour of Euglena on a 2D fast rotating clinostat and a 3D clinostat as well as under real microgravity conditions (TEXUS sounding rocket flight), of Paramecium on a 2D clinostat and in micro-gravity. The data show similar results during 2D and 3D clinorotation compared to real microgravity with respect to loss of orientation (gravitaxis) of Paramecium and Euglena and a decrease of linearity of the cell tracks of Euglena. However, the increase of the mean swimming velocities, especially during 3D clinorotation (Euglena) and 2D clinorotation of Paramecium might indicate a persisting mechanostimulation of the cells. Further studies including long-term 2D and 3D clinostat exposition will enable us to demonstrate the qualification of the applied simulation methods.


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    Title :

    Comparative studies on gravisensitive protists on ground (2D and 3D clinostats) and in microgravity


    Additional title:

    Vergleichende Untersuchung von gravitationsempfindlichen Protisten in terrestrischer Umgebung (2D- und 3D-Klinostat) und unter Mikrogravitätsbedingungen


    Contributors:


    Publication date :

    2006


    Size :

    3 Seiten, 2 Bilder, 12 Quellen




    Type of media :

    Conference paper


    Type of material :

    Print


    Language :

    English




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