To provide fusion proteins capable of binding to oil droplets while maintaining enzymatic activity to synthesize hydrophobic compounds, methods for producing materials using the fusion proteins to accumulate hydrophobic compounds in oil droplets, vectors capable of improving the yield of the hydrophobic compound by introducing the vector into cells by using genetic engineering, and transformed cells into which the vector or a gene encoding the fusion protein has been introduced.SOLUTION: This invention relates to a fusion protein comprising: a first amino acid sequence that can bind to oil droplets (lipid droplets); and a second amino acid sequence having an enzymatic activity to synthesize a hydrophobic compound. The first amino acid sequence and the second amino acid sequence are fused via a linker sequence (third amino acid sequence) and the enzymatic activity of the second amino acid sequence is maintained.SELECTED DRAWING: Figure 1
【課題】疎水性化合物を合成する酵素活性を有しつつ、油滴に結合可能な融合タンパク質、該融合タンパク質を用いて、疎水性化合物を油滴内に蓄積させる物質製造方法、遺伝子組換え技術により細胞に導入することで疎水性化合物の生産量を向上させることができるベクター、該ベクター若しくは該融合タンパク質をコードする遺伝子が導入された形質転換細胞を提供することを目的とする。【解決手段】油滴(lipid droplet)に結合可能なアミノ酸配列(第1アミノ酸配列)と、疎水性化合物を合成する酵素活性を有するアミノ酸配列(第2アミノ酸配列)とを有し、前記第1アミノ酸配列と第2アミノ酸配列とがリンカー配列(第3アミノ酸配列)を介して融合しており、第2アミノ酸配列の酵素活性が保持されている融合タンパク質。【選択図】図1
FUSION PROTEINS, MATERIAL PRODUCTION METHODS, VECTORS, TRANSFORMED CELLS, PNEUMATIC TIRE PRODUCTION METHODS, AND RUBBER PRODUCT PRODUCTION METHODS
融合タンパク質、物質製造方法、ベクター、形質転換細胞、空気入りタイヤの製造方法及びゴム製品の製造方法
2021-02-12
Patent
Electronic Resource
Japanese
IPC: | C12N Mikroorganismen oder Enzyme , MICROORGANISMS OR ENZYMES / B29D PRODUCING PARTICULAR ARTICLES FROM PLASTICS OR FROM SUBSTANCES IN A PLASTIC STATE , Herstellen besonderer Gegenstände aus Kunststoff oder aus Stoffen in plastischem Zustand / B60C VEHICLE TYRES , Fahrzeugreifen / C07K PEPTIDES , Peptide / C08C TREATMENT OR CHEMICAL MODIFICATION OF RUBBERS , Behandlung oder chemische Modifizierung von Kautschuken / C12P FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE , Gärungsverfahren oder Verfahren unter Verwendung von Enzymen zur gezielten Synthese von chemischen Verbindungen oder Zusammensetzungen oder zur Trennung optischer Isomerer aus einer racemischen Mischung |
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