Novel non-invasive micro-CT contrast agent for quantitative virtual 3D histology of mineralized and soft skeletal tissues

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Novel non-invasive micro-CT contrast agent for quantitative virtual 3D histology of mineralized and soft skeletal tissues

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Kerckhofs, Greet / Stegen, Steve / Van Gastel, N / Sap, Annelies / Falgayrac, G / Penel, G / Durand, M / Luyten, Frank / Geris, Liesbet / Vandamme, Katleen / ... [more]

Abstract

Organs and biological tissues have a complex and heterogeneous 3D structure, and thus 2D measurements like histomorphometry only partially reveal the full 3D structure. As a solution, we propose contrast-enhanced microfocus computed tomography (CE-CT) for quantitative virtual 3D histology of multiple tissues in a single dataset. As most existing contrast agents are invasive, toxic and/or non-specific, we show the non-invasive character of a novel contrast agent that allows to simultaneously visualize the bone and bone marrow compartment, including the vascularization and adiposity, in murine long bones. Moreover, we have quantified the 3D structure of these different tissues in different mouse models (i.e. ageing and type 2 diabetes), showing the added value of CE-CT compared to standard histomorphometry. The additional information that CE-CT is providing compared to standard histomorphometry, with a spatial dimension, could bring novel insights in the biological processes during tissue development, remodeling and regeneration.

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Title :

Novel non-invasive micro-CT contrast agent for quantitative virtual 3D histology of mineralized and soft skeletal tissues

Contributors:

Kerckhofs, Greet (author) / Stegen, Steve (author) / Van Gastel, N (author) / Sap, Annelies (author) / Falgayrac, G (author) / Penel, G (author) / Durand, M (author) / Luyten, Frank (author) / Geris, Liesbet (author) / Vandamme, Katleen (author)

Publication date :

2017-01-01

Type of media :

Conference paper

Type of material :

Electronic Resource

Language :

English

Keywords :

contrast enhanced computed tomography (CE-CT) , virtual 3D histology , skeletal tissues , 3D visualization & quantification

Classification :

DDC: