A rough road vibration stimulus was reconstructed on a shaker platform to verify if seat effective amplitude transmissibility (SEAT) values can be used as a reliable metric to determine dynamic seat comfort using seven seats and six subjects. The virtual seat method was combined with a paired comparison procedure to obtain reliable subjective dynamic seat comfort data. The psychometric method of constants, a 1-up-1-down Levitt procedure and a 2-up-l-down Levitt procedure were compared experimentally to find the most accurate and efficient paired comparison scheme. A two-track, interleaved, 2-up-l-down Levitt procedure was used for the subjective dynamic seat comfort assessment. SEAT value is an objective metric and has been widely used to determine seat vibration isolation efficiency. There was excellent correlation (R² = 0.97) between the subjective ratings and estimated SEAT values on the seat-top when the values are averaged over the six subjects. This study suggests that SEAT values, estimated from averaged seat-top transmissibility functions of six carefully selected subjects, could be used as a reliable metric to select the best seat for a specific road vibration input. The six subjects who participated in this study were carefully selected to comprise a profile that represents the general population. The results show a significant improvement in the correlation between measured and estimated SEAT values and subjective-objective dynamic seat comfort, when individual values are averaged over the test subjects. Van Niekerk et al. reported a similar improvement in the correlation of averaged results for six subjects selected according to the same criterion. The correlation in this study was found to be R² = 0.97 compared to 0.94, albeit for only seven seats versus the 16 seats in the previous study. Including the seatback vibration did not improve the correlation. The conclusions of this study suggest that the SEAT values, estimated from averaged seat-top transmissibility of six carefully selected subjects, can be used to select the best seat for a specific road vibration input.