We present images of human skin obtained by a two-photon fluorescence microscope, which has been optimized for in- vivo imaging. Using autofluorescence as a contrast mechanism various layers of the skin, e.g., stratum corneum, viable epidermis, basal layer and upper dermis could be clearly distinguished. A comparison between two-photon fluorescence images and reflectance images shows that there is a clear difference in the information content provided by both techniques. In particular, the fluorescence images of the dermis show detail that is absent in the reflectance images.
Two-photon fluorescence microscopy of in-vivo human skin
Laser Microscopy ; 2000 ; Amsterdam,Netherlands
Proc. SPIE ; 4164
2000-12-18
Aufsatz (Konferenz)
Elektronische Ressource
Englisch
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