Abstract This study proposes a flexible and scalable single-level framework for origin–destination matrix (ODM) inference using data from IoT (Internet of Things) and other sources. The framework allows the analyst to integrate information from multiple data sources, while controlling for differences in data quality across sources. We assess the effectiveness of the framework through a real-world experiment in Greater Adelaide (GA), Australia. We infer car OD flows within the region using four separate data sources: site-level traffic counts from loop detectors, vehicle trajectories recorded by roadside Bluetooth sensors, partial OD flows based on data from in to vehicle navigation systems, and journey-to-work OD data collected by the Australian Census. We compare our OD inferences with those from the current version of the Strategic Adelaide Model (SAM), calibrated using data from traditional household travel surveys. We find remarkable consistency between our inferences and those from SAM, despite differences in input data and methodologies. For example, for the morning peak period, we predict the total number of trips made within GA to be equal to 556,000, while the corresponding prediction from SAM is equal to 484,000. The two predictions are within 15 per cent of each other. When we compare the spatial distribution of trips, in terms of origins and destinations, we find that our inferred OD matrix has an 86 per cent cosine similarity to the corresponding SAM matrix. Travel time predictions based on our OD inferences correspond closely to those predicted by the Google Directions API, lending further confidence in our inferences. In summary, our results show that the proposed framework can produce highly comparable ODM, trip production and trip attraction patterns to those inferred from traditional household travel survey-based transportation demand modelling methods.