In order to improve performance of internal combustion engines and meet the requirements of the new pollutant emission regulations, advanced combustion strategies have been investigated. The newly designed partially premixed combustion concept has demonstrated its potential for reducing NOx and particulate matter emissions combined with high indicated efficiencies while still retaining proper control over combustion process by using different injection strategies. In this study, parametric variations of injection pressure, second injection and third injection timings were experimentally performed to analyze the effect of the injection strategy over the air/fuel mixture process and its consequent impact on gaseous compound emissions and particulate matter emissions including its size distribution. Tests were carried out on a newly designed two-stroke high-speed direct injection compression-ignition engine operating with the partially premixed combustion concept using 95 research octane number gasoline fuel. A scanning particle sizer was used to measure the particles size distribution and the HORIBA 7100DEGR gas analyzer system to determine gaseous emissions. Three different steady-state operation modes in terms of indicated mean effective pressure and engine speed were investigated: 3.5 bar indicated mean effective pressure and 2000 r/min, 5.5 bar indicated mean effective pressure and 2000 r/min, and 5.5 bar indicated mean effective pressure and 2500 r/min. The experimental results confirm how the use of an adequate injection strategy is indispensable to obtain low exhaust emissions values and a balance between the different pollutants. With the increase in the injection pressure and delay in the second injection, it was possible to obtain a trade-off between NOx and particulate matter emission reduction, while there was an increase in hydrocarbon and carbon monoxide emissions under these conditions. In addition, the experiments showed an increase in particle number emissions and a progressive shift in the particles size distribution toward larger sizes, increasing the accumulation-mode particles and reducing the nucleation-mode particles with the decrease in the injection pressure and delay in the third injection.