SCIFLI, The Scientifically Calibrated In-Flight Imagery (https://scifli.larc.nasa.gov/), team at NASA Langley Research Center specializes in the collection of multispectral data for space vehicles during Ascent and Entry, Descent, and Landing (EDL) observations. Multispectral datasets are invaluable across the NASA Agency and to commercial stakeholders for evaluating the performance of launch and re-entry space vehicles and ensuring the safety of the scientific research community. The launch of Artemis 1 marked the initiation of NASA returning to lunar exploration. SCIFLI’s Airborne Multispectral Imager (SAMI) was deployed by the SCIFLI team to scientifically document the historic event and provide the NASA research community with aerial footage of the event in wavelength channels ranging from the ultraviolet (UV) to the visible (VIS) to mid-wave infrared (MWIR). SAMI was designed to spectrally image unique aerothermal phenomena during the Artemis 1 launch. The use of these datasets captured in-flight during the launch will provide insight to research organizations across the Agency; aiding in validation efforts for simulations and modeling that contributed to the kickoff of the Agency’s resurgence to lunar exploration. This presentation will focus on the imagery captured on SAMI by the WB-57 team on November 16th, 2022, during the observation. Imaging objectives were identified and considered beforehand to determine the configuration of the SAMI instrument for the imaging mission. SCIFLI and Opto-Knowledge Systems, Inc. (OKSI) performed a thorough review of the datasets collected to identify and characterize aerothermal phenomena occurring during the observation. Additional post-processing was completed to provide quantitatively calibrated temperature images of the rocket during the observation. Some of the candidates were not optimal for quantitative temperature extraction due to common degradation factors, but after various image enhancements they proved useful for qualitatively characterizing different phenomena during the observation.